Abstract
EPR spectroscopy of full-length vimentin and X-ray crystallography of vimentin peptides has provided concordant structural data for nearly the entire central rod domain of the protein. In this report, we use a combination of EPR spectroscopy and molecular modeling to determine the structure and dynamics of the missing region and unite the separate elements into a single structure. Validation of the L1-2 modeling approach is demonstrated by the close correlation between EPR and X-ray data in the previously solved regions. Importantly, Molecular Dynamics (MD) simulation of the constructed model agrees with spin label motion as determined by EPR. Furthermore, MD simulation shows L1-2 heterogeneity, with a concerted switching of states among the dimer chains. These data provide the first ever experimentally- driven model of a complete IF rod domain, providing research tools for further modeling and assembly studies.
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