Complete sequence-specific 1H nuclear magnetic resonance assignments for the α-amylase polypeptide inhibitor tendamistat from Streptomyces tendae

Abstract

The 1H nuclear magnetic resonance (n.m.r.) spectrum of the α-amylase inhibitor Tendamistat was completely assigned with the use of phase-sensitive homonuclear twodimensional n.m.r. The assignments include the non-labile protons of the 74 amino acid residues as well as the labile protons which exchange sufficiently slowly to be observed in H 2O solution. The proton chemical shifts are listed at 50 °C and pH 3.2, which coincides with the conditions used for the determination of the three-dimensional structure of Tendamistat.