Complete or High-Quality Draft Genome Sequences of Six Xanthomonas hortorum Strains Sequenced with Short- and Long-Read Technologies.

Nay C Dia,Joël F Pothier,Fabio Rezzonico,Theo H M Smits,David A Baltrus

doi:10.1128/mra.00828-20

Nay C Dia, Joël F Pothier + Show 3 more

Open Access

https://doi.org/10.1128/mra.00828-20

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Abstract

We report the genome sequences of six Xanthomonas hortorum species-level clade members, X. hortorum pathovars taraxaci, pelargonii, cynarae, and gardneri (complete genome sequences) and X. hortorum pathovars carotae and vitians (high-quality draft genome sequences). Both short- and long-read sequencing technologies were used.

Highlights

The genetic relatedness of the Xanthomonas hortorum species-level clade was established by Parkinson et al using partial gyrB gene sequences (1)
The strains were initially obtained as freeze-dried cultures in glass ampoules from two international strain collections abbreviated in the strain names as CFBP (Collection Française de Bactéries Associées aux Plantes, Beaucouzé, France) and NCPPB (National Collection of Plant Pathogenic Bacteria, York, United Kingdom)
After revival on nutrient yeast extract glycerol agar plates (4) for 2 days at 28°C, an isolated colony was streaked onto the same medium and grown in the same manner

Summary

Introduction

The genetic relatedness of the Xanthomonas hortorum species-level clade (slc) was established by Parkinson et al using partial gyrB gene sequences (1). The strains were initially obtained as freeze-dried cultures in glass ampoules from two international strain collections abbreviated in the strain names as CFBP (Collection Française de Bactéries Associées aux Plantes, Beaucouzé, France) and NCPPB (National Collection of Plant Pathogenic Bacteria, York, United Kingdom). Genomic DNA (gDNA) for Illumina MiSeq short-read sequencing was extracted from cells grown overnight at 28°C in nutrient yeast extract glycerol broth (4) using the NucleoSpin tissue kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol with the following specifications: elution buffer was heated at 70°C before use, and the gDNA was eluted with 60 ␮l of this buffer.

Results

Conclusion