Abstract
A virus closely related to Cherry green ring mottle virus (CGRMV) was isolated from a tree displaying typical symptoms of cherry necrotic rusty mottle disease. We have named this virus Cherry necrotic rusty mottle virus (CNRMV) and report here its complete genomic sequence as determined from overlapping cloned cDNAs. CNRMV has a genome of 8,432 nucleotides excluding the 3’ poly(A) sequence and codes for 7 significant open reading frames (ORFs). Five of these ORFs are conserved among all fovea-, allexi-, potex- and carlaviruses and code for a methyltransferase/ helicase/polymerase polypeptide, the triple gene block movement proteins, and the coat protein. Two further ORFs, ORFs 2a and 5a, are nested completely within ORFs 2 and 5, respectively. The putative translation products from these ORFs display sequence similarity with putative translation products from two similarly nested ORFs present in the CGRMV genome. The function of these two ORFs is unknown, nor are they conserved among other related viruses. Attempts to mechanically transmit the virus to a herbaceous host failed.
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