Abstract
A complementary DNA copy of the 3a cistron of dicistronic RNA3 from brome mosaic virus has been cloned and sequenced. Based on this and other data, the complete nucleotide sequence of brome mosaic virus RNA3 is presented. Following a 91 base 5′ non-coding region, the 909 base 3a cistron encodes a protein of predicted molecular weight 32,480. Between the 3a and coat protein cistrons is an approximately 250 base intercistronic non-coding region. Overlapping the intercistronic region by 9 bases, the last 876 bases of RNA3 encode the subgenomic coat protein messenger, RNA4, completely and without interruption. A third long open reading frame of possible evolutionary significance overlaps the 3a and coat protein cistrons. An internal poly(A) of heterogeneous length (16 to 22 bases) occurs in the intercistronic region just 20 bases 5′ to the start of RNA4 sequences. Averaging the poly(A) heterogeneity, the length of brome mosaic virus RNA3 is 2114 bases.
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