Complete Nucleotide Sequence of a Partitivirus from Rhizoctonia solani AG-1 IA Strain C24.

Chen Liu,Erxun Zhou,Canwei Shu,Miaolin Zeng,Meiling Zhang

doi:10.3390/v10120703

Abstract

The complete genome of a novel double-stranded (ds) RNA mycovirus, named as Rhizoctonia solani partitivirus 5 (RsPV5), isolated from rice sheath blight fungus R. solani AG-1 IA strain C24, was sequenced and analysed. RsPV5 consists of two segments, dsRNA-1 (1899 nucleotides) and dsRNA-2 (1787 nucleotides). DsRNA-1 has an open reading frame (ORF) 1 that potentially codes for a protein of 584 amino acid (aa) containing the conserved motifs of a RNA-dependent RNA polymerase (RdRp), and dsRNA-2 also contains a ORF 2, encoding a putative capsid protein (CP) of 513 aa. Phylogenetic analysis revealed that RsPV5 clustered together with six other viruses in an independent clade of the genus Alphapartitivirus, indicating that RsPV5 was a new member of the genus Alphapartitivirus, within the family Partitiviridae.

Highlights

Mycoviruses are widely distributed in fungi, of which, only a few affect their fungal hosts resulting in alterations of growth rate or enhanced virulence or hypovirulence [1,2]
Phylogenetic analysis revealed that Rhizoctonia solani partitivirus 5 (RsPV5) clustered together with six other viruses in an independent clade of the genus Alphapartitivirus, indicating that RsPV5 was a new member of the genus Alphapartitivirus, within the family Partitiviridae
In this study we describe the complete nucleotide sequence of another partitivirus nominated Rhizoctonia solani partitivirus 5 (RsPV5), isolated from R. solani AG-1 IA strain C24

Summary

Introduction

Mycoviruses (fungal viruses or viruses of fungi) are widely distributed in fungi, of which, only a few affect their fungal hosts resulting in alterations of growth rate or enhanced virulence or hypovirulence [1,2]. Mycoviruses with hypovirulent traits are anticipated to be important biological control agents against plant fungal diseases in the future [3]. A wide range of mycoviruses, such as Rosellinia necatrix partitivirus. 1-W8 [5], Botrytis cinerea mitovirus 1 [6], and Rhizoctonia solani partitivirus 2 [7], have been discovered in different fungi. Mycoviruses are mainly classified into 14 families, including. RNA (-ssRNA) viruses (Mycomononegaviridae) and family Amalgamaviridae [8,9]. Many mycoviruses remain unclassified [8], and the first circular ssDNA mycovirus from the phytopathogenic fungus Sclerotinia sclerotiorum was discovered in 2010 [10]

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Conclusion