Abstract
Manis javanica has been listed as a critically endangered species by IUCN due to over commercial harvesting (for food and medicine) and international trade. Identifying the species and sources of M. javanica in markets is important for resource conservation and trade controls. Mitochondrial DNA is a powerful molecular marker for species identification, phylogeography analysis, population genetics research and evolutionary studies. In this study, we sequenced and characterized the complete mitochondrial genome of M. javanica. The full length of the complete mitochondrial DNA was 16,576 bp. It contained 13 protein-coding genes, 22 tRNA genes and two rRNA genes. The tRNA genes fold into the expected cloverleaf secondary structures with normal base pairing, except the tRNASer(AGN) which lost the stem of DHC loop. This investigation provides complete mitochondrial genome of M. javanica which is an effective molecular tool for the genetic research and protection of this endangered species.
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