Abstract

In this study, we report the complete mitochondrial genome of the Grayish Mouse Opossumm, Tlacuatzin canescens. The mitochondrial genome is 16 460 bp in length and has a base composition of A (35.7%), T (31.3%), C (21.3%), and G (11.7%), demonstrating an obvious bias of high AT content (67%). The mitochondrial genome contains a typically conserved structure, encoding 13 protein-coding genes (PCGs), 20 transfer RNA genes (tRNA), 2 ribosomal RNA genes (12S rRNA and 16S rRNA), and a control region (D-loop region). Except for ND6 gene, all other PCGs were located on the H-strand. ND4 gene and ND4L gene were overlapped by 4 bp, and ATP8 gene and ATP6 gene were overlapped by 45 bp. The nucleotide sequence of 13 PCGs of T. canescens and other 30 Didelphimorphia species were used for phylogenetic analysis. The result indicated T. canescens a relative close relationship with species Monodelphis domestica.

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