Abstract

The complete mitochondrial genome of the Sakhalin nine-spined stickleback Pungitius tymensis was determined using Illumina paired-end sequencing of genomic DNA. The genome sequence was 16 481 bp in length, consisting of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and a control region. The content and arrangement of the genes were identical to those of other Gasterosteidae species. P. tymensis was phylogenetically positioned with other Pungitius species (P. kaibarae, P. pungitius and P. sinensis) with a clear distinction from them. Nucleotide identity in the 37 genic regions ranged from 94.7% to 94.9% between P. tymensis and the other Pungitius species.

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