Abstract
Stoneflies comprise an ancient group of insects, but the phylogenetic position of Plecoptera and phylogenetic relations within Plecoptera have long been controversial, and more molecular data is required to reconstruct precise phylogeny. Herein, we present the complete mitogenome of a stonefly, Suwallia teleckojensis, which is 16146 bp in length and consists of 13 protein-coding genes (PCGs), 2 ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs) and a control region (CR). Most PCGs initiate with the standard start codon ATN. However, ND5 and ND1 started with GTG and TTG. Typical termination codons TAA and TAG were found in eleven PCGs, and the remaining two PCGs (COII and ND5) have incomplete termination codons. All transfer RNA genes (tRNAs) have the classic cloverleaf secondary structures, with the exception of tRNASer(AGN), which lacks the dihydrouridine (DHU) arm. Secondary structures of the two ribosomal RNAs were shown referring to previous models. A large tandem repeat region, two potential stem-loop (SL) structures, Poly N structure (2 poly-A, 1 poly-T and 1 poly-C), and four conserved sequence blocks (CSBs) were detected in the control region. Finally, both maximum likelihood (ML) and Bayesian inference (BI) analyses suggested that the Capniidae was monophyletic, and the other five stonefly families form a monophyletic group. In this study, S. teleckojensis was closely related to Sweltsa longistyla, and Chloroperlidae and Perlidae were herein supported to be a sister group.
Highlights
In metazoans, the mitochondrial genome is usually a circular, double-stranded molecule, ranging in size from 13 to 16 kb [1,2]
We provided the second mitogenome of the stonefly family Chloroperlidae to facilitate a study of mitochondrial phylogeny in the Plecoptera
We reconstructed the phylogenetic tree of S. teleckojensis and other stoneflies based on protein coding genes (PCGs), our result increases the understanding of stonefly phylogeny
Summary
The mitochondrial genome (mitogenome) is usually a circular, double-stranded molecule, ranging in size from 13 to 16 kb [1,2]. An A + T-rich region is known as the non-coding region or the control region (CR), which is involved in the initiation of transcription and replication [2] Because of their abundance, small size, fast rate of evolution, and low levels of sequence recombination, mitochondrial genomes are increasingly applied to comparative and molecular evolution, phylogenetic studies, and population genetics [3]. The Plecoptera (stoneflies) is a small order of hemimetabolous insects It is comprised of 16 families and includes about 3900 described species worldwide [4,5]. We provided the second mitogenome of the stonefly family Chloroperlidae to facilitate a study of mitochondrial phylogeny in the Plecoptera. We reconstructed the phylogenetic tree of S. teleckojensis and other stoneflies based on PCGs, our result increases the understanding of stonefly phylogeny
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