Complete mitochondrial genome of Ostrinia palustralis memnialis Walker, 1859 (Lepidoptera: Crambidae)

Abstract

The complete mitochondrial genome (mitogenome) of Ostrinia palustralis memnialis Walker, 1859 (Lepidoptera: Crambidae) was determined to be 15,246 bp with a typical set of genes (13 protein-coding genes [PCGs], 2 rRNA genes, and 22 tRNA genes) and one non-coding region, with an arrangement identical to that observed in most lepidopteran genomes. Twelve PCGs have the typical ATN start codon, whereas COI has an atypical CGA codon frequently found in the start region of the lepidopteran COI. At 330 bp, the A + T-rich region is well within the range found in other Pyraloidea. The A + T content of the whole genome, PCGs, srRNA, lrRNA, tRNAs, and the A + T-rich region all are well within the range found in other Pyraloidea. Phylogenetic analyses of the concatenated sequences of the 13 PCGs using Bayesian inference (BI) and maximum-likelihood (ML) methods placed O. palustralis as a sister group to Ostrinia furnacalis and Ostrinia nubilalis, with the highest nodal support. The subfamilies within Crambidae, such as Nymphulinae, Spilomelinae, and Pyraustinae, all formed monophyletic groups with the highest nodal support.