Abstract
Datong horse processes a muscular physique with high tolerance to extensive management and adapts well to high altitude conditions in Qinghai, China. In this study, the whole mitochondrial genome sequence of E. caballus determined using next-generation sequencing data was 16,654 bp in length, consisting of 13 protein-coding genes, two rRNA genes, 22 tRNA genes, and a non-coding control region. Notably, there was one tandem repeat with 21 ‘CACCTGTG’ located in the control region. The base composition of the genome was A (32.3%), C (28.6%), G (13.3%), and T (25.8%), with an A + T content of 58.1%. Molecular phylogeny demonstrated that E. caballus was more closely related to a breed from central Asia and processed distinct relationships with some Chinese breeds. This work characterized the mitogenome of E. caballus (Datong horse breed) and present significant genetic information for this valuable specimen.
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