Complete mitochondrial genome of Capnia zijinshana (Plecoptera: Capniidae) and phylogenetic analysis among stoneflies

Abstract

The complete mitochondrial genome (mitogenome) of the winter stonefly, Capnia zijinshana (Plecoptera: Capniidae), was sequenced and compared with other stoneflies. The mitogenome of C. zijinshana is 16,310bp long and contains 37 genes including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (CR). The gene arrangement of C. zijinshana and other 12 stonefly mitogenomes is identical with the putative ancestral mitogenome in Drosophila yakuba. Twenty-one of the 22 tRNAs in the C. zijinshana mitogenome could be folded into the typical cloverleaf secondary structure, while the dihydrouridine (DHU) arm of trnS (AGN) was missing. The 1513-bp control region of C. zijinshana contained two potential stem-loop structures and a tandem repeat region. The phylogenetic relationships of 13 species from Plecoptera were analyzed based on the nucleotide sequences of 13 PCGs. The phylogenetic relationships among 13 stoneflies and two mayflies were inferred by Bayesian inference and maximum likelihood methods. C. zijinshana was grouped with another two winter stoneflies, Mesocapnia arizonensis and Apteroperla tikumana. The two analyses both supported the close relationship of Pteronarcyidae and Chloroperlidae, and also supported the sister-group relationship of Styloperlidae and Perlidae.