Abstract
The distribution of the linear α-1,4-linked unit chains in rabbit-liver glycogen CL 14) and oyster glycogen ( ovbar| CL 12) were investigated mainly using Pseudomonas isoamylase. This enzyme cleaved α-1,6-glucosidic branching linkages extensively, to form linear α-1,4-linked unit chains of DP 15 from rabbit-liver and of DP 11 from oyster glycogen. The products were fractionated by Sephadex G-75 chromatography, giving one peak with a fairly broad distribution of chain-lengths ranging from 3 to about 50 glucose units. Pullulanase released maltosaccharides of DP 7–8 from glycogen, leaving approximately 70% of resistant limit dextrins. The branches in pullulanase limit dextrins from rabbit-liver and oyster glycogens were broken completely by Pseudomonas isoamylase to yield linear chains of DP 21 and 16 , respectively. The branching pattern in the glycogen molecule is discussed on the basis of these findings.
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