Abstract
Clostridium limosum can be found in soil and the intestinal tract of animals. In 2014, C. limosum was isolated from a suspected blackleg outbreak in cattle in Schleswig-Holstein, Germany. We present a complete genome sequence of a C. limosum strain represented by a circular chromosome and three plasmids.
Highlights
Clostridium limosum can be found in soil and the intestinal tract of animals
The C. limosum isolate (14S0207) was cultured in 3 ml Selzer broth (6) under anaerobic conditions followed by genomic DNA extraction using a Genomic-tip 100/Q kit (Qiagen, Germany)
The raw reads were checked for quality before and after read trimming using FastQC version 0.11.7
Summary
Clostridium limosum can be found in soil and the intestinal tract of animals. In 2014, C. limosum was isolated from a suspected blackleg outbreak in cattle in Schleswig-Holstein, Germany. GATC Biotech (Germany) carried out genome sequencing using a PacBio RS II sequencer (7), including the preceding library preparation to create a 10to 20-kb insert size library. The total number of reads was 43,854 with an average length of 15,204 bp. A total of 1,067,720 reads were received.
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