Abstract
The complete genomic sequences of H5N1 highly pathogenic avian influenza (HPAI) viruses were directly obtained from lung, trachea, and colon tissues and an intestinal fecal sample of a patient by using the next-generation sequencing technique. This is the first report on complete H5N1 viral genomes from human autopsy specimens.
Highlights
The H5N1 highly pathogenic avian influenza Orthomyxoviridae
Autopsy samples were collected by a pathologist using the standard technique for routine histologic analysis
Total RNA was extracted from all specimens by using TRIzol reagent (Invitrogen, Carlsbad, CA) and was further subjected to cDNA synthesis by using the SuperScript III first-strand synthesis system (Invitrogen)
Summary
The H5N1 highly pathogenic avian influenza Orthomyxoviridae. It is an enveloped virus (HPAI) virus belongs to the with an eight-segmented, family singlestranded RNA genome of negative polarity, with a length of about 13.5 kb in total. Autopsy samples were collected by a pathologist using the standard technique for routine histologic analysis. These samples were stored frozen at Ϫ80°C until used. Total RNA was extracted from all specimens by using TRIzol reagent (Invitrogen, Carlsbad, CA) and was further subjected to cDNA synthesis by using the SuperScript III first-strand synthesis system (Invitrogen).
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