Abstract
Clostridium perfringens (Cp) is a ubiquitous opportunistic pathogen of humans and animals in the natural environment and animal intestines. The pathogenicity of Cp depends on the production of toxins encoded by genes on the chromosomes or plasmids. In contemporary literature, there is no clear consensus about the pathogenicity of CpA β2 toxin. To analyze the homology of the genome of piglet source CpA and its β2 toxin, we sequenced the whole genome of strain JXJA17 isolated from diarrhea piglets using the Illumina Miseq and Pacbio Sequel platforms. The genome was composed of a circular chromosome with 3,324,072 bp (G + C content: 28.51%) and nine plasmids. Genome and 16S rDNA homology analysis revealed a close relation of the JXJA17 strain with the JGS1495, Cp-06, Cp-16, and FORC_003 strains. These strains were isolated from different samples and belonged to different toxin-types. JXJA17 strain was found to carry two toxin genes (plc and cpb2). In contrast to other Cp strains, the cpb2 of JXJA17 was located on a large plasmid (58 kb) with no co-localization of other toxin genes or antibiotic resistance genes. Analysis of JXJA17 genome homology and its cpb2 would facilitate our further study the relationship between β2 toxin and piglet diarrhea.
Highlights
Clostridium perfringens is a ubiquitous Gram-positive, rod-shaped anaerobic b acterium[1]
There is a paucity of data related to the complete genome sequences of Clostridium perfringens type A (CpA) isolated from diarrheal piglets
The results indicated no significant correlation of the strains with the host sources, toxinotypes, or geographical distribution
Summary
Clostridium perfringens is a ubiquitous Gram-positive, rod-shaped anaerobic b acterium[1]. Strain JXJA17 was one of 229 Cp isolated from the rectal contents of neonatal piglets with diarrhea and was identified as CpA by toxin typing. This strain carries cpb[2], which is more pathogenic than the CpA isolated from healthy piglets not carrying cpb[2], based on animal experiments of mouse toxicity[12] and rabbit ileal loop model. For better characterization β2 toxin of CpA isolated from piglet with diarrhea, we sequenced the JXJA17 complete genome and analyzed the cpb[2]
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