Abstract
Predominant community-associated methicillin-resistant Staphylococcus aureus strain USA300 is believed to have originated from an ancestral methicillin-susceptible strain, although the details of that evolution remain unknown. To help understand the emergence of this highly successful strain, we sequenced the genomes of two methicillin-susceptible Staphylococcus aureus clinical strains that are very closely related to USA300.
Highlights
Predominant community-associated methicillin-resistant Staphylococcus aureus strain USA300 is believed to have originated from an ancestral methicillinsusceptible strain, the details of that evolution remain unknown
Contig assembly was done using the RS Hierarchical Genome Assembly Process (HGAP) protocol version 2.3.0.140936.p5 [15,16,17], with read quality controlled by aligning short reads on longer reads using BLASR [15]
Two contigs were assembled for methicillin-susceptible S. aureus (MSSA) strain C3948 from 85,037 raw reads, covering 1,142,835,600 sequenced bases, with an N50 value of 2,823,074 bp
Summary
Predominant community-associated methicillin-resistant Staphylococcus aureus strain USA300 is believed to have originated from an ancestral methicillinsusceptible strain, the details of that evolution remain unknown. Genomic DNA was isolated by phenol-chloroform extraction of overnight cultures started from a single colony. DNA sequencing, contig assembly, and genome circularization were performed at the Génome Québec Innovation Centre in Montreal, Canada.
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