Complete Genome Sequences of Three of the Earliest Community-Associated Methicillin-Resistant Staphylococcus aureus Strains Isolated in Remote Western Australia.

Nicola M Karakatsanis,Elena Colombi,Joshua P Ramsay,Geoffrey W Coombs,Shakeel Mowlaboccus,Julie C Pearson

doi:10.1128/mra.00797-21

Abstract

ABSTRACTInitially reported in Western Australia in the 1980s, community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has become a major cause of S. aureus infections globally. We report the complete genome sequences of three of the earliest CA-MRSA strains isolated from remote Australian Indigenous communities in the Kimberley region of Western Australia.

Highlights

Isolated S. aureus strains [4] were rehydrated from lyophilized stocks in tryptic soy broth (TSB) and grown overnight on blood agar at 37°C
The Oxford Nanopore Technologies (ONT) SQKRAD004 kit was used to create DNA sequencing libraries, which were loaded onto an ONT FLO-MIN106 flow cell and sequenced using ONT MinKNOW v20.10.3 and MinKNOW Core v4.1.2
Base calling was performed with Guppy v4.4.111c81d62 (ONT), using the dna_r9.4.1_450bps_hac.cfg model

Summary

Introduction

Isolated S. aureus strains [4] were rehydrated from lyophilized stocks in tryptic soy broth (TSB) and grown overnight on blood agar at 37°C. Genomes were sequenced using the MinION Mk1B system (ONT, UK). The ONT SQKRAD004 kit was used to create DNA sequencing libraries, which were loaded onto an ONT FLO-MIN106 flow cell and sequenced using ONT MinKNOW v20.10.3 and MinKNOW Core v4.1.2.

Results

Conclusion