Complete Genome Sequences of the p1 Gene Type 2b and 2c Strains Mycoplasma pneumoniae KCH-402 and KCH-405.

Tsuyoshi Kenri,Hiroyuki Fujii,Keigo Shibayama,Hitomi Ohya,Hiroshi Nakajima,Masato Suzuki,Atsuko Horino,Tsuyoshi Sekizuka,Toru Hashimoto,Makoto Kuroda

doi:10.1128/genomea.00513-17

Abstract

ABSTRACTHere, we present the complete genome sequences of Mycoplasma pneumoniae KCH-402 and KCH-405, which are p1 gene type 2b and 2c strains, respectively. These strains harbor variations in the orf6 gene, which encodes the cytadherence-related proteins P40 and P90.

Highlights

We present the complete genome sequences of Mycoplasma pneumoniae KCH-402 and KCH-405, which are p1 gene type 2b and 2c strains, respectively
Assembled contigs were mapped on the type 2a reference genome of strain 309
Annotation was performed manually based on the gene nomenclature of reference strains M129

Summary

Introduction

We present the complete genome sequences of Mycoplasma pneumoniae KCH-402 and KCH-405, which are p1 gene type 2b and 2c strains, respectively. We extracted genomic DNA from KCH-402 and KCH-405 cultured in PPLO medium (Becton Dickinson, Sparks, MD, USA) using the QIAamp DNA mini kit (Qiagen, Hilden, Germany). Genome sequencing was performed using the Illumina HiSeq 4000 platform. Assembled contigs were mapped on the type 2a reference genome of strain 309

Results

Conclusion