Complete Genome Sequences of Novel Bovine T4, rv5-Like, and Dhillonviruses Effective against Escherichia coli O157.

Domonkos Sváb,István Tóth,Trinad Chakraborty,Linda Falgenhauer,John J Dennehy

doi:10.1128/mra.01261-20

Abstract

Enterohemorrhagic Escherichia coli (EHEC) isolates of serotype O157:H7 are serious foodborne zoonotic pathogens and prime targets for biocontrol using bacteriophages. We report on the complete genome sequences of 11 novel lytic bacteriophages, representing three viral genera, isolated from cattle in Hungary that target E. coli O157 strains.

Highlights

The role of bacteriophages as alternative antimicrobial agents has gained renewed interest in the past years [1]
Quality control and clipping of the reads were performed within the framework of the ASA3P pipeline [10]
SRR13132432 SRR13132431 SRR13132431 SRR13132430 SRR13132429 SRR13132428 SRR13132430 SRR13132427 SRR13132429 SRR13132428 SRR13132426 a nt, nucleotides. b These phages were isolated from the same environmental sample. c These phages were isolated from the same bovine fecal sample. d These phages were isolated from the same bovine fecal sample

Summary

Introduction

The role of bacteriophages as alternative antimicrobial agents has gained renewed interest in the past years [1]. Phage DNA from active lytic stocks was isolated using the phenol-chloroform method [9] and concentrated with the DNA Clean and Concentrator kit (Zymo Research, CA, USA) according to the manufacturer’s instructions. Genomic DNA sequencing libraries were prepared using the Nextera XT kit (Illumina, Eindhoven, Netherlands). Sequencing was performed using the NextSeq midoutput reagent kit v2.5 (2 Â 150 bp) on an Illumina NextSeq 500 machine.

Results

Conclusion