Abstract
We report 6 draft genome sequences corresponding to Mycobacterium tuberculosis H37Rv and M. tuberculosis DR689, a Beijing isolate, plus their counterparts subjected to 200 continuous passages in Middlebrook 7H9 broth, either alone or with ox bile.
Highlights
Tuberculosis is still a public health problem worldwide (1)
DNA was extracted from the colonies grown on Lowenstein-Jensen agar using the cetyltrimethylammonium bromide (CTAB)-NaCl technique with modifications described previously (6)
The libraries were sequenced on a NextSeq 500 instrument using midoutput v2 chemistry (2 ϫ 150 bp) or on a MiSeq instrument using V2 chemistry (2 ϫ 250 bp); the fastq reads obtained were mapped against the M. tuberculosis H37Rv reference genome sequence reported in GenBank using the BWA-MEM aligner (7) from the Sequencher v5.4.6 suite
Summary
Tuberculosis is still a public health problem worldwide (1). In Mexico, the number of new pulmonary and meningeal tuberculosis cases has increased in the last several years (2). DNA was extracted from the colonies grown on Lowenstein-Jensen agar using the cetyltrimethylammonium bromide (CTAB)-NaCl technique with modifications described previously (6). High-throughput sequencing was carried out using the Illumina MiSeq genome analyzer (San Diego, CA, USA).
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