Abstract
Here, we present the complete genome sequences of Mycobacterium smegmatis phages Chewbacca, Reptar3000, and Riparian, isolated from soil in Las Vegas, NV. The phages were isolated and annotated by undergraduate students enrolled in the Phage Discovery course offered by the School of Life Sciences at the University of Nevada, Las Vegas.
Highlights
We present the complete genome sequences of Mycobacterium smegmatis phages Chewbacca, Reptar3000, and Riparian, isolated from soil in Las Vegas, NV
Phages were assigned to clusters based on genomic sequence similarity using the PhagesDB.org database and the Phamerator software with default settings [1, 4]
A ClustalW multiple alignment using default settings showed that all three phage pairs have roughly 40% average nucleotide sequence identity (ANI) between them, which is the same as that of two randomly generated DNA sequences
Summary
We present the complete genome sequences of Mycobacterium smegmatis phages Chewbacca, Reptar3000, and Riparian, isolated from soil in Las Vegas, NV. Phages were assigned to clusters based on genomic sequence similarity using the PhagesDB.org database and the Phamerator software with default settings [1, 4]. Chewbacca is a member of cluster N; Reptar3000, cluster K4; and Riparian, cluster R.
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