Abstract
Carbapenemase-producing Enterobacterales, including KPC-2 producers, have become a major clinical problem. During an outbreak in Quebec City, Canada, KPC-2-producing Klebsiella michiganensis and Citrobacter farmeri were isolated from a patient six weeks apart. We determined their complete genome sequences. Both isolates carried nearly identical IncN2 plasmids with blaKPC-2 on a Tn4401b element. Both strains also carried IncP1 plasmids, but that of C. farmeri did not carry a Beta-lactamase gene, whereas that of K. michiganensis carried a second copy of blaKPC-2 on Tn4401b. These results suggest recent plasmid transfer between the two species and a recent transposition event.
Highlights
Carbapenemase-producing enterobacteria (CRE) have become a major problem throughout the world
We report here the sequences of the chromosomes, the four plasmids of C. farmeri, and the two plasmids of K. michiganensis, which are very similar to two of the plasmids of C. farmeri, except that both K. michiganensis plasmids carry Tn4401b and encode blaKPC-2, whereas only one of the C. farmeri plasmids does
The complete genome sequences of Klebsiella michiganensis CCRI-24235 and Citrobacter farmeri CCRI-24236 were determined by PacBio and polished with Illumina to resolve homopolymer undercounts
Summary
Carbapenemase-producing enterobacteria (CRE) have become a major problem throughout the world. KPC carbapenemases are plasmid-mediated and often found on a Tn4401b transposon [2,3]. KPC has spread throughout the United States and into Canada, with outbreaks in Toronto, Montreal, and Quebec City [4,5]. We obtained two strains identified as KPC producers from the Hôtel-Dieu hospital in Quebec City, where five KPC-producing strains were isolated in 2017 from four patients on four different floors. These two strains were anal swab isolates that colonized but did not infect the patient, a 69-year-old male. We report here the sequences of the chromosomes, the four plasmids of C. farmeri, and the two plasmids of K. michiganensis, which are very similar to two of the plasmids of C. farmeri, except that both K. michiganensis plasmids carry Tn4401b and encode blaKPC-2, whereas only one of the C. farmeri plasmids does
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