Abstract
Here, we present complete genome sequences of four Enterococcus faecium isolates, obtained from two patients with apparent vancomycin-resistant Enterococcus faecium bacteremia; these isolates also carried two mutations known to be associated with daptomycin resistance. Sequences were obtained using de novo and hybrid assembly of Oxford Nanopore and Illumina sequence data.
Highlights
We present complete genome sequences of four Enterococcus faecium isolates, obtained from two patients with apparent vancomycin-resistant Enterococcus faecium bacteremia; these isolates carried two mutations known to be associated with daptomycin resistance
The vancomycin-resistant Enterococcus faecium (VREfm) isolates from positive blood cultures were subcultured on blood agar plates
Each genomic DNA sample was subdivided into two aliquots; one was subjected to Oxford Nanopore Technologies (ONT) library preparation and the other to Illumina library preparation
Summary
Complete Genome Sequences of Four Isolates of VancomycinResistant Enterococcus faecium with the vanA Gene and Two Daptomycin Resistance Mutations, Obtained from Two Inpatients with Prolonged Bacteremia. Piroon Jenjaroenpun,a Thidathip Wongsurawat,a Zulema Udaondo,a Courtney Anderson,b James Lopez,b Meera Mohan,b Ruslana Tytarenko,c Brian Walker,c Intawat Nookaew,a David Ussery,a Atul Kothari,b Se-Ran Juna aDepartment of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA bDivision of Infectious Diseases, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA cMyeloma Institute, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA. Piroon Jenjaroenpun and Thidathip Wongsurawat contributed to this work.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
