Abstract
We report the complete genome sequencing and annotation of four Salmonella enterica serovar Enteritidis isolates, two that are representative of the Central/Eastern African clade (CP255 and D7795) and two of the Global Epidemic clade (A1636 and P125109).
Highlights
Salmonella enterica serovar Enteritidis typically causes gastroenteritis and is responsible for a global epidemic linked to poultry and egg production
DNA integrity was verified by 0.5% agarose gel electrophoresis at 90 V for 1.5 h
DNA purity/concentration were measured with a DeNovix DS-11FX spectrophotometer/ fluorometer and Qubit double-stranded DNA high-sensitivity (HS) assay kit
Summary
Salmonella enterica serovar Enteritidis typically causes gastroenteritis and is responsible for a global epidemic linked to poultry and egg production. A single colony of each isolate was grown for 16 h in 5 ml of Lennox medium at 37°C. Total DNA was extracted using the Quick-DNA universal kit (Zymo; catalog number D4069). Long-read sequencing was performed by the Centre for Genomic Research (University of Liverpool, UK) in a PacBio single-molecule real-time (SMRT) cell (P6/C4 chemistry) using SMRTbell Template v1.0 (Pacific Biosciences; catalog number 100-259-100) library preparation with g-TUBE (Covaris) fragmentation and size selection of 15 to 50 kb with 0.75% agarose cassette (BluePippin; catalog number BMF7510).
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