Abstract
In 2011, the Association of Analytical Communities (AOAC) International released a list of Bacillus strains relevant to biothreat molecular detection assays. We present the complete and annotated genome assemblies for the 15 strains listed on the inclusivity panel, as well as the 20 strains listed on the exclusivity panel.
Highlights
For several years the idea that biothreat and contamination detection methods need to be better characterized has been discussed [1,2,3]
Consensus sequences from Hierarchical Genome Assembly Process (HGAP) and Allpaths were computationally shredded into 10kbp overlapping pieces
All but one of the genomes were assembled into finished-quality complete genomes [11]
Summary
For several years the idea that biothreat and contamination detection methods need to be better characterized has been discussed [1,2,3]. This led the Association of Analytical Communities (AOAC) International to compose a bacterial strain list for evaluation when designing molecular detection assays. This list, termed the Stakeholder Panel on Agent Detection Assays (SPADA), includes 15 inclusivity and 20 exclusivity Bacillus strains [4]. As testing pertaining to these strains involves nucleic acid analyses, complete genome assemblies can further improve assays confidence, a major issue in both positive and negative results [5].
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