Abstract
Vibrio anguillarum causes a fatal hemorrhagic septicemia in marine fish that leads to great economical losses in aquaculture world-wide. Vibrio anguillarum strain NB10 serotype O1 is a Gram-negative, motile, curved rod-shaped bacterium, isolated from a diseased fish on the Swedish coast of the Gulf of Bothnia, and is slightly halophilic. Strain NB10 is a virulent isolate that readily colonizes fish skin and intestinal tissues. Here, the features of this bacterium are described and the annotation and analysis of its complete genome sequence is presented. The genome is 4,373,835 bp in size, consists of two circular chromosomes and one plasmid, and contains 3,783 protein-coding genes and 129 RNA genes.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0060-7) contains supplementary material, which is available to authorized users.
Highlights
Vibrio anguillarum is a marine pathogen that causes a fatal hemorrhagic septicemia, termed vibriosis, in cultured and wild fish as well as in mollusks and crustaceans [1, 2]
Since horizontal transfer of DNA is a common method for acquiring new DNA in strains, we screened the three genomes using similar parameters for genomic islands and prophages using the IslandViewer and Phage search tool (PHAST) search tools and the presence of integrons was determined by identifying attC sites, a feature of small mobile gene cassettes that are captured by integrons [45]
Genome comparisons were done with the complete genomes of two other virulent, O1 serotype V. anguillarum strains, M3 and 775
Summary
Vibrio anguillarum is a marine pathogen that causes a fatal hemorrhagic septicemia, termed vibriosis, in cultured and wild fish as well as in mollusks and crustaceans [1, 2]. Using the Basic Local Alignment Search Tool [23], homology searches of all CDSs were done against the Clusters of Orthologous Groups database [24] enabling the assignment of COG functional categories to the CDSs. The tRNAscan-SE 1.21 tool [25, 26] was used to identify tRNA genes; the RNAmmer 1.2 program [27] was used to identify rRNA genes; and the Rfam database [28] and manual curation was used to identify other non-coding RNAs. Term Completed 400-600-bp fragments (454), >10-kb fragments (PacBio) Roche 454 Life Sciences Pacific Biotechnologies PacBio 60 × Staden-gap, Newbler (Roche/454 GS FLX), SMRTanalysis, version 2.0.1, HGAP module (Celera and Quiver) Glimmer, tRNAscan-SE 1.21, Rfam, RNAmmer VANGcI, VANGcII, VANGp67 GCA000786425 September 1, 2014 Gp0102007 PRJEB5701 NB10 Aquaculture, fish pathogen
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