Abstract
Campylobacter jejuni strain PT14 is a clinical isolate previously used to propagate bacteriophages in the United Kingdom phage typing scheme. The strain has proven useful in the isolation of Campylobacter bacteriophages from several sources, and it functions as a model host in phage therapy experiments with poultry and poultry meat.
Highlights
Campylobacter jejuni strain PT14 is a clinical isolate previously used to propagate bacteriophages in the United Kingdom phage typing scheme
C. jejuni PT14 contains 26 probable pseudogenes, but notably, it has three annotated pseudogenes from C. jejuni strain NCTC 11168, Cj0501, Cj1064, and Cj1470c, which remain intact within the PT14 genome
The clustered regularly interspaced short palindromic repeats and associated genes (CRISPR-Cas) within the genome of C. jejuni PT14 were found to be intact, which is an interesting finding considering the sensitive nature of this strain to bacteriophage infections
Summary
Campylobacter jejuni strain PT14 is a clinical isolate previously used to propagate bacteriophages in the United Kingdom phage typing scheme. The genome sequence of C. jejuni PT14 was determined by pyrosequencing on a 454 GS FLX platform (Roche Diagnostics). A total of 257,645 reads were generated, with an average read length of 352 bases. The sequence was compared and confirmed with 5 million 50-bp reads generated using MiSeq technology operating in paired-end mode (Illumina).
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