Abstract
Bacterial species belonging to the genus Pseudobutyrivibrio are important members of the rumen microbiome contributing to the degradation of complex plant polysaccharides. Pseudobutyrivibrio xylanivorans MA3014 was selected for genome sequencing to examine its ability to breakdown and utilize plant polysaccharides. The complete genome sequence of MA3014 is 3.58 Mb, consists of three replicons (a chromosome, chromid, and plasmid), has an overall G + C content of 39.6%, and encodes 3,265 putative protein-coding genes (CDS). Comparative pan-genomic analysis of all cultivated and currently available P. xylanivorans genomes has revealed a strong correlation of orthologous genes within this rumen bacterial species. MA3014 is metabolically versatile and capable of growing on a range of simple mono- or oligosaccharides derived from complex plant polysaccharides such as pectins, mannans, starch, and hemicelluloses, with lactate, butyrate, and formate as the principal fermentation end products. The genes encoding these metabolic pathways have been identified and MA3014 is predicted to encode an extensive range of Carbohydrate-Active enZYmes with 78 glycoside hydrolases, 13 carbohydrate esterases, and 54 glycosyl transferases, suggesting an important role in solubilization of plant matter in the rumen.
Highlights
Butyrivibrio and Pseudobutyrivibrio represent the most commonly isolated butyrate-producing anaerobic rumen bacteria (Henderson, et al 2015), and are among the small number of rumen genera capable of utilizing the complex plant structural polysaccharide xylan (Bryant and Small1956; Hungate 1966)
Xylanivorans MA3014, a strain isolated from a New Zealand pasture-grazed dairy cow (Seshadri, et al 2018), and describe its comparison with other representative P. xylanivorans genomes
P. xylanivorans MA3014 was isolated from the rumen contents of fistulated Friesian dairy cattle and sequenced (Noel 2013; Seshadri, et al 2018)
Summary
Butyrivibrio and Pseudobutyrivibrio represent the most commonly isolated butyrate-producing anaerobic rumen bacteria (Henderson, et al 2015), and are among the small number of rumen genera capable of utilizing the complex plant structural polysaccharide xylan Pseudobutyrivibrio [family Lachnospiraceae, order Clostridiales] are anaerobic, monotrichous, butyrate-producing, curved rods and have been isolated from the gastrointestinal tracts of various ruminants, monogastric animals and humans (Kopecný, et al.2003; Willems and Collins 2009). To accommodate the observed diversity amongst the newly discovered bacterial strains, a new genus, Pseudobutyrivibrio, was described in which only P. ruminis and P. xylanivorans species are currently recognized (Kopecný, et al 2003; Van. Gylswyk, et al 1996). Xylanivorans MA3014, a strain isolated from a New Zealand pasture-grazed dairy cow (Seshadri, et al 2018), and describe its comparison with other representative P. xylanivorans genomes
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
