Complete Genome Sequence of the Plant Pathogen Ralstonia solanacearum Strain CIAT-078, Isolated in Colombia, Obtained Using Oxford Nanopore Technology.

Diana López-Alvarez,Viviana Dominguez,Ana M Leiva,Israel Barrantes,Wilmer J Cuellar,Juan M Pardo,Christina A Cuomo

doi:10.1128/mra.00448-20

Abstract

Moko is one of the main diseases affecting banana and plantain in Colombia. Here, we report the genome sequence of the causal agent, the bacterium Ralstonia solanacearum (Smith) strain CIAT-078, collected in 2004 from affected plantains in central-west Colombia. The assembled genome was obtained using Oxford Nanopore Technology.

Highlights

Ralstonia solanacearum (Smith) belongs pathogenic bacteria that colonize the to a species xylem tissue complex of of hundreds soilborne of plant phytospecies worldwide [1]
We selected CIAT-078, a pathogenic strain collected in 2004 from Moko-affected fields (Quindio, Colombia) that was previously characterized at the pathogenicity and biochemical level [7, 8] and used in routine screening for resistance assays [6]
CIAT-078 was reactivated in semiselective medium South Africa (SMSA) [9] for 4 days and in nutrient agar (Difco, USA) for 48 hours at 28°C

Summary

Introduction

Ralstonia solanacearum (Smith) belongs pathogenic bacteria that colonize the to a species xylem tissue complex of of hundreds soilborne of plant phytospecies worldwide [1]. We selected CIAT-078, a pathogenic strain collected in 2004 from Moko-affected fields (Quindio, Colombia) that was previously characterized at the pathogenicity and biochemical level [7, 8] and used in routine screening for resistance assays [6]. CIAT-078 was reactivated in semiselective medium South Africa (SMSA) [9] for 4 days and in nutrient agar (Difco, USA) for 48 hours at 28°C. The bacteria were grown in LB medium prior to DNA extraction

Results

Conclusion