Abstract
Subcluster F1 bacteriophage KingMidas was isolated from soil collected in Providence, Rhode Island, using Mycobacterium smegmatis mc2155 as the host. The genome is 57,386 bp and contains 105 predicted protein-coding genes but no transfer-messenger RNAs or tRNAs. This siphovirus has an icosahedral head, with a genome 99.1% identical to that of F1 mycobacteriophage Scottish.
Highlights
Subcluster F1 bacteriophage KingMidas was isolated from soil collected in Providence, Rhode Island, using Mycobacterium smegmatis mc2155 as the host
Annotation was performed using the PECAAN workflow tool [5], and completed files were transferred to DNA Master v5.22.23
KingMidas includes genes for proteins that may regulate life cycle events and transcription, as well as genes for two glycosyltransferases and proteins that may act in DNA synthesis, modification, or repair (DNAQ-like DNA polymerase III subunit, Ku-like double-stranded DNA break binding protein, three DNA methylases, an exonuclease, two HNH endonucleases, and RecA-like DNA recombinase)
Summary
Subcluster F1 bacteriophage KingMidas was isolated from soil collected in Providence, Rhode Island, using Mycobacterium smegmatis mc2155 as the host. Annotation was performed using the PECAAN workflow tool [5], and completed files were transferred to DNA Master v5.22.23 (https://phagesdb.org/DNAMaster). PECAAN resources to identify putative genes included GLIMMER v3.0 [6], GeneMark v2.5 [7], Phamerator [8], Starterator v1.1 (https://seaphages.org/software), ARAGORN v1.2.38 [9], and tRNAscan-SE v3.0 [10].
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