Complete genome sequence of the bacteriochlorophyll a-containing Roseibacterium elongatum type strain (DSM 19469(T)), a representative of the Roseobacter group isolated from Australian coast sand.

Thomas Riedel,Anne Fiebig,Hans-Peter Klenk,Markus Göker

doi:10.4056/sigs.5541028

Abstract

Roseibacterium elongatum Suzuki et al. 2006 is a pink-pigmented and bacteriochlorophyll a-producing representative of the Roseobacter group within the alphaproteobacterial family Rhodobacteraceae. Representatives of the marine ‘Roseobacter group’ were found to be abundant in the ocean and play an important role in global and biogeochemical processes. In the present study we describe the features of R. elongatum strain OCh 323T together with its genome sequence and annotation. The 3,555,102 bp long genome consists of one circular chromosome with no extrachromosomal elements and is one of the smallest known Roseobacter genomes. It contains 3,540 protein-coding genes and 59 RNA genes. Genome analysis revealed the presence of a photosynthetic gene cluster, which putatively enables a photoheterotrophic lifestyle. Gene sequences associated with quorum sensing, motility, surface attachment, and thiosulfate and carbon monoxide oxidation could be detected. The genome was sequenced as part of the activities of the Transregional Collaborative Research Centre 51 (TRR51) funded by the German Research Foundation (DFG).

Highlights

Strain OCh 323T (= DSM 19469T = CIP 107377T = JCM 11220T) is the type strain of Roseibacterium elongatum in the bispecific genus Roseibacterium [1] with R. beibuensis [2] being the second species in the genus
We present a description of the genome sequencing and annotation and a summary classification together with a set of features for strain DSM 19469T, including novel aspects of its phenotype and features of the organism
Genome analysis of strain R. elongatum DSM 19469T revealed the presence of genes putatively associated with Quorum sensing (QS) like a N-acyl-L-homoserine lactone synthetase (LuxI homolog; roselon_01555) and a regulator of the LuxR family

Summary

Introduction

Whole genome sequencing of strain R. elongatum DSM 19469T revealed a complete and finished genome size of 3,555,109 bp, which seems to be the smallest completed genome of representatives of the Roseobacter group up to date [46]. In the genome sequence of R. elongatum DSM 19469T several putative phageassociated gene sequences were detected, organized in gene clusters (e.g., roselon_02355 - 02370). Genome analysis of strain R. elongatum DSM 19469T revealed the presence of genes putatively associated with QS like a N-acyl-L-homoserine lactone synthetase (LuxI homolog; roselon_01555) and a regulator of the LuxR family (roselon_3097).

Results

Conclusion