Abstract
Serratia marcescens ATCC 274 produces the red pigment prodigiosin and the biosurfactant serrawettin W1 at 30°C but not at 37°C. A complete, high-quality genome sequence of S. marcescens ATCC 274 was obtained and found to comprise a single 5,148,533-bp circular genome with 4,799 genes.
Highlights
Serratia marcescens ATCC 274 produces the red pigment prodigiosin and the biosurfactant serrawettin W1 at 30°C but not at 37°C
Five hundred nanograms of the same genomic DNA was used for the Illumina library and was enzymatically fragmented to produce 300- to 350-bp-long DNA fragments using the Nextera DNA Flex library preparation kit (Illumina, CA) according to the manufacturer’s instructions
The final complete genome consisted of 5,148,533 bp, with a GϩC content of 59.5% and 105ϫ
Summary
Serratia marcescens ATCC 274 produces the red pigment prodigiosin and the biosurfactant serrawettin W1 at 30°C but not at 37°C. Five hundred nanograms of the same genomic DNA was used for the Illumina library and was enzymatically fragmented to produce 300- to 350-bp-long DNA fragments using the Nextera DNA Flex library preparation kit (Illumina, CA) according to the manufacturer’s instructions. Paired-end (2 ϫ 150-bp) reads (3,550,000 reads with an average read length of 153 bp) were obtained with the MiSeq system (Illumina), and raw reads were filtered (Ͻ10 bp and/or ϽQ30) using fastp v.0.14.1 software (7), resulting in a total of 3,198,000 reads with an average read length of 148 bp.
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