Complete Genome Sequence of Southern tomato virus Identified in China Using Next-Generation Sequencing.

Chellappan Padmanabhan,Rugang Li,Shu-E Sun,Deyong Zhang,Zhangjun Fei,Kai-Shu Ling,Yong Liu,Yi Zheng

doi:10.1128/genomea.01226-15

Abstract

The complete genome sequence of Southern tomato virus (STV), a double-stranded RNA virus that affects tomato in China, was determined using small RNA deep sequencing. This Chinese isolate shares 99% sequence identity to other isolates from Mexico, France, Spain, and the United States. This is the first report of STV infecting tomatoes in Asia.

Highlights

The complete genome sequence of Southern tomato virus (STV), a double-stranded RNA virus that affects tomato in China, was determined using small RNA deep sequencing
STV appears to have a high rate of seed transmission, but with no apparent evidence of graft or mechanical transmission (1)
To determine the causal agent(s), total RNA was prepared with TRIzol reagent on a pooled sample collected from eight diseased tomato plants

Summary

Introduction

The complete genome sequence of Southern tomato virus (STV), a double-stranded RNA virus that affects tomato in China, was determined using small RNA deep sequencing. Southern tomato virus (STV), which causes tomato mosaic and yellow stunting disorder, was first characterized for its presence in Mexico and the United States (1). STV is a double-stranded RNA (dsRNA) virus with a small genome of approximately 3.5 kb (1). STV appears to have a high rate of seed transmission, but with no apparent evidence of graft or mechanical transmission (1).

Results

Conclusion