Abstract
Serratia marcescens is an opportunistic human pathogen with multiple resistance mechanisms that infects hospitalized patients. Here, we report the full genome sequence of S. marcescens podophage Parlo. Parlo is most similar to Erwinia phage PEp14 and encodes a 3,764-residue protein assumed to be a homolog of DarB, an antirestriction protein.
Highlights
Serratia within marcescens is a Gram-negative opportunistic human many environments and is well known for infecting pathogen [1]
Parlo grows on Serratia marcescens D1 (Ward’s Science, catalog no. 8887172) and was isolated from pooled swine, fecal, and soil samples collected at private farms primarily around Bryan and College Station, Texas
Hosts and phages were cultured at 30°C in LB broth and agar (BD) as previously described [3]
Summary
Serratia within marcescens is a Gram-negative opportunistic human many environments and is well known for infecting pathogen [1]. Hosts and phages were cultured at 30°C in LB broth and agar (BD) as previously described [3]. Phage morphology was determined using a JEOL 1200EX transmission electron microscope in the Texas A&M Microscopy and Imaging Center to observe samples negatively stained with 2% (wt/vol) uranyl acetate [4]. Quality checks and trimming were performed on the 373,956 total reads using FastQC
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