Abstract
Salmonella enterica serovar Pullorum is a chicken-adapted pathogen, causing pullorum disease. Its strict host adaptation has been suspected to result in gene decay. To validate this hypothesis and identify the decayed genes, we sequenced the complete genome of S. Pullorum RKS5078. We found 263 pseudogenes in this strain and conducted functional analyses of the decayed genes.
Highlights
Salmonella enterica serovar Pullorum is a chicken-adapted pathogen, causing pullorum disease
The genomic DNA was sheared into 3-kb fragments by the Hydroshear instrument and was sequenced on a SOLiD sequencer by the mate-pair strategy (2 ϫ 50 bp), as detailed in the manual for the instrument (Applied Biosystems)
The relationships between contigs were determined following the physical map published earlier (3), and the gaps between contigs were closed by PCR amplification of the genomic DNA and sequencing
Summary
Salmonella enterica serovar Pullorum is a chicken-adapted pathogen, causing pullorum disease. It is very closely related to another chicken pathogen, Salmonella enterica serovar Gallinarum, and, to a lesser degree, to a host generalist, Salmonella enterica serovar Enteritidis. Pullorum has the special genetic and pathogenic features suitable for studies to elucidate the genetic basis and evolution of host adaptation and acquisition of novel pathogenicity in Salmonella.
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