Abstract
Here, we report the closed genome sequence of Clostridium aceticum, an Rnf- and cytochrome-containing autotrophic acetogen that is able to convert CO2 and H2 to acetate using the Wood-Ljungdahl pathway. The genome consists of a circular chromosome (4.2 Mbp) and a small circular plasmid (5.7 kbp).
Highlights
We report the closed genome sequence of Clostridium aceticum, an Rnf- and cytochrome-containing autotrophic acetogen that is able to convert CO2 and H2 to acetate using the Wood-Ljungdahl pathway
The Integrated Microbial GenomesExpert Review (IMG-ER) system [9] was used for automatic annotation, which was subsequently manually curated by using the Swiss-Prot, TrEMBL, and InterPro databases [10]
The genome harbored 6 rRNA cluster, 74 tRNA genes, 3,181 protein-coding genes with predicted functions, and 743 genes coding for hypothetical proteins
Summary
We report the closed genome sequence of Clostridium aceticum, an Rnf- and cytochrome-containing autotrophic acetogen that is able to convert CO2 and H2 to acetate using the Wood-Ljungdahl pathway. Clostridium aceticum, an anaerobic and endospore-forming organism, was the first bacterial isolate described to be able to form acetate autotrophically from a CO2 ϩ H2 gas mixture. This organism is able to grow heterotrophically on sugars, organic acids, and alcohols.
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