Abstract
Here, we report the annotated whole-genome sequence of Pseudomonas sp. strain SK, isolated in India from organic wheat rhizosphere. This strain has proved to be a species with potential biocontrol activity against soilborne plant pathogens based on antiSMASH analysis.
Highlights
The fluorescent Pseudomonas strains present in rhizospheric soil play a critical role by combating fungal and bacterial pathogenic stress through the production of several metabolites and antibiotics (1)
Pseudomonas sp. strain SK was isolated by serial dilution of rhizospheric soil samples, followed by plating onto specific Pseudomonas isolation agar base (HiMedia, India) with overnight incubation at 30°C
To confirm that this strain belongs to the genus Pseudomonas, 16S rRNA and rpoD gene sequencing were carried out using the following primer sets: universal primers for 16S rRNA genes 8F (5=-AGAGTTTGATCCTGGCTCAG-3=) and 1492R (5=-GGTTACCTTGTTACGACTT-3=) and the rpoD gene primers PsEG30F (5=-A TYGAAATCGCCAARCG-3=) and PsEG790R (5=-CGGTTGATKTCCTTGA-3=)
Summary
The fluorescent Pseudomonas strains present in rhizospheric soil play a critical role by combating fungal and bacterial pathogenic stress through the production of several metabolites and antibiotics (1). Strain SK was isolated by serial dilution of rhizospheric soil samples, followed by plating onto specific Pseudomonas isolation agar base (HiMedia, India) with overnight incubation at 30°C. To confirm that this strain belongs to the genus Pseudomonas, 16S rRNA and rpoD gene sequencing were carried out using the following primer sets: universal primers for 16S rRNA genes 8F (5=-AGAGTTTGATCCTGGCTCAG-3=) and 1492R (5=-GGTTACCTTGTTACGACTT-3=) and the rpoD gene primers PsEG30F (5=-A TYGAAATCGCCAARCG-3=) and PsEG790R (5=-CGGTTGATKTCCTTGA-3=).
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