Abstract
Pseudomonas fluorescens strain PICF7 is a native endophyte of olive roots. Previous studies have shown this motile, Gram-negative, non-sporulating bacterium is an effective biocontrol agent against the soil-borne fungus Verticillium dahliae, the causal agent of one of the most devastating diseases for olive (Olea europaea L.) cultivation. Here, we announce and describe the complete genome sequence of Pseudomonas fluorescens strain PICF7 consisting of a circular chromosome of 6,136,735 bp that encodes 5,567 protein-coding genes and 88 RNA-only encoding genes. Genome analysis revealed genes predicting factors such as secretion systems, siderophores, detoxifying compounds or volatile components. Further analysis of the genome sequence of PICF7 will help in gaining insights into biocontrol and endophytism.
Highlights
Pseudomonas fluorescens PICF7 is a native colonizer of olive (Olea europaea L.) roots and an in vitro antagonist of the soil-borne fungal phytopathogen Verticillium dahliae Kleb. [1], the causal agent of Verticillium wilts in a large number of plant species [2]
Strain PICF7 is able to display an endophytic lifestyle within olive root tissues under different experimental conditions [3,5,6] and induces a broad range of defence responses at both local and systemic level, as well as to activate diverse transcription factors known to be involved in systemic defence responses [7,8]
In this report we describe the complete genome sequence of Pseudomonas fluorescens strain PICF7, a “Pseudomonadales” in the order Gammaproteobacteria that was originally isolated from the roots of healthy nursery-produced olive plants cv
Summary
Pseudomonas fluorescens PICF7 is a native colonizer of olive (Olea europaea L.) roots and an in vitro antagonist of the soil-borne fungal phytopathogen Verticillium dahliae Kleb. [1], the causal agent of Verticillium wilts in a large number of plant species [2]. Genome project history P. fluorescens strain PICF7 was selected for sequencing due to its ability to exert biocontrol against Verticillium wilt of olive [1,3] and to develop an endophytic lifestyle within olive root tissues [5,6]. Growth conditions and DNA isolation P. fluorescens strain PICF7 was grown in 50 ml of LB medium and incubated for 16 h at 28°C After this period of time, the OD600 of the culture was 1.2. A gene predicting a salycilic hydroxylase has been identified in PICF7 genome This gene could be involved in the degradation of the plant defence hormone salicylic acid, disrupting the systemic response against colonizing bacteria.
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