Abstract
Pseudomonas aeruginosa is an opportunistic pathogen that is able to cause various infections, including airway infections in cystic fibrosis patients. Here, we present the complete closed and annotated genome sequence of P. aeruginosa AA2, an isolate obtained early during infection of the respiratory tract of a German cystic fibrosis patient.
Highlights
Pseudomonas aeruginosa is an opportunistic pathogen that is able to cause various infections, including airway infections in cystic fibrosis patients
Libraries were prepared with the NEBNext kit (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform [7]
For PacBio sequencing, DNA extraction was performed with the Wizard genomic DNA purification kit (Promega)
Summary
Pseudomonas aeruginosa is an opportunistic pathogen that is able to cause various infections, including airway infections in cystic fibrosis patients. Cells were grown in liquid culture overnight, inoculated directly from Ϫ80°C frozen stock to minimize the number of passages, and pelleted by centrifugation. For Illumina sequencing, DNA was extracted using bead beating [7]. Libraries were prepared with the NEBNext kit (New England Biolabs) and sequenced on the Illumina NextSeq 500 platform [7]. A total of 5,350,348 reads (150 bp) were obtained, 96.9% of which were paired.
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