Abstract
ABSTRACTThe complete genome sequence of a variant of porcine deltacoronavirus, isolated from a diarrheal piglet and designated CH/JXJGS01/2016, was sequenced and analyzed. Phylogenetic analysis demonstrated that CH/JXJGS01/2016 shares the highest nucleotide and amino acid identities with the Chinese strain NH (GenBank accession number KU981059).
Highlights
Porcine deltacoronavirus (PDCoV) is an enveloped single-stranded positive-sense RNA virus that belongs to the newly classified genus Deltacoronavirus, in the family Coronaviridae [1,2,3]
A homology analysis was carried out based on the full-length genome sequence and S gene of CH/JXJGS01/2016 and the reference strains of PDCoV retrieved from GenBank
The results revealed that CH/JXJGS01/2016 had the closest relationship to and highest nucleotide identity (99.4%) with a Chinese PDCoV strain, NH (GenBank accession number KU981059), and had the lowest nucleotide identity with two strains from Thailand
Summary
Porcine deltacoronavirus (PDCoV) is an enveloped single-stranded positive-sense RNA virus that belongs to the newly classified genus Deltacoronavirus, in the family Coronaviridae [1,2,3]. The viral RNA was extracted from the suspensions of virus cultured on a pig kidney cell line (LLC-PK1), and the whole-genome sequence of CH/JXJGS01/2016 was amplified and sequenced based on the methods that we previously reported [8]. Sequences were assembled into the full-length genome of CH/JXJGS01/2016 using Lasergene version 7.0 software (DNAStar, Inc., Madison, WI, USA).
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