Complete Genome Sequence of Pantoea ananatis Strain NN08200, an Endophytic Bacterium Isolated from Sugarcane

Quan Zeng,Zemei Nong,Xuelian Ye,Guoying Shi,Chunjin Hu

doi:10.1007/s00284-020-01972-x

Quan Zeng, Zemei Nong + Show 3 more

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https://doi.org/10.1007/s00284-020-01972-x

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Journal: Current Microbiology	Publication Date: Apr 3, 2020
Citations: 8	License type: open-access

Affiliation: Guangxi Academy of Agricultural Science

Abstract

Stain NN08200 was isolated from the surface-sterilized stem of sugarcane grown in Guangxi province of China. The stain was Gram-negative, facultative anaerobic, non-spore-forming bacteria. The complete genome SNP-based phylogenetic analysis indicate that NN08200 is a member of the genus Pantoea ananatis. Here, we summarize the features of strain NN08200 and describe its complete genome. The genome contains a chromosome and two plasmids, in total 5,176,640 nucleotides with 54.76% GC content. The chromosome genome contains 4598 protein-coding genes, and 135 ncRNA genes, including 22 rRNA genes, 78 tRNA genes and 35 sRNA genes, the plasmid 1 contains 149 protein-coding genes and the plasmid 2 contains 308 protein-coding genes. We identified 130 tandem repeats, 101 transposon genes, and 16 predicted genomic islands on the chromosome. We found an indole pyruvate decarboxylase encoding gene which involved in the biosynthesis of the plant hormone indole-3-acetic acid, it may explain the reason why NN08200 stain have growth-promoting effects on sugarcane. Considering the pathogenic potential and its versatility of the species of the genus Pantoea, the genome information of the strain NN08200 give us a chance to determine the genetic background of interactions between endophytic enterobacteria and plants.

Highlights

The genus Pantoea comprises several species that are associated with plants have been found, either as pathogenic or beneficial bacteria to plants [1, 2]
Sequence determinations and phylogenetic analysis of the 16S Ribosomal RNA (rRNA) gene indicated that strain NN08200 is affiliated with the genus Pantoea, and the strain was preserved in the China General Microbiological Culture Collection Center, with the preservation number CGMCC No 5438
A PHYML method phylogenetic tree based on SNP of complete genomes for strain belonging to the genus Pantoea constructed by TreeBeST (Fig. 2) showed that strain NN08200 is most closely related to strains belonging to the Pantoea ananatis [20]

Summary

Introduction

The genus Pantoea comprises several species that are associated with plants have been found, either as pathogenic or beneficial bacteria to plants [1, 2]. Sequence determinations and phylogenetic analysis of the 16S rRNA gene indicated that strain NN08200 is affiliated with the genus Pantoea, and the strain was preserved in the China General Microbiological Culture Collection Center, with the preservation number CGMCC No 5438. Complete Genome Sequence of Pantoea ananatis Strain NN08200, an Endophytic Bacterium Isolated...

Results

Conclusion