Complete Genome Sequence of Pandoraea pnomenusa TF-18, a Multidrug-Resistant Organism Isolated from the Rhizosphere of Rice (Oryza sativa L. subsp. japonica).

Mihnea R Mangalea,Bradley R Borlee,Jan E Leach,Janet Ziegle,Emily K Luna,Richard A Bowen,Christine Chang,Angela M Bosco-Lauth,David Rasko

doi:10.1128/mra.01008-19

Abstract

Pandoraea pnomenusa strain TF-18 was isolated from the roots of rice seedlings on selective medium containing four classes of antibiotics for isolation of Burkholderia pseudomallei Using Pacific Biosciences (PacBio) single-molecule real-time (SMRT) sequencing technology, we report here a complete genome of 5,499,432 bases, a GC content of 64.8%, and 4,849 coding sequences.

Highlights

Pandoraea pnomenusa strain TF-18 was isolated from the roots of rice seedlings on selective medium containing four classes of antibiotics for isolation of Burkholderia pseudomallei
Genomic DNA was extracted from a representative isolate, P. pnomenusa TF-18
Individual colonies were grown on LB agar with no antibiotics at 28°C for 2 days and suspended in phosphate-buffered saline (PBS)

Summary

Introduction

Pandoraea pnomenusa strain TF-18 was isolated from the roots of rice seedlings on selective medium containing four classes of antibiotics for isolation of Burkholderia pseudomallei. Genomic DNA was extracted from a representative isolate, P. pnomenusa TF-18. Genomic DNA was purified using phenolchloroform-isoamyl alcohol (25:24:1), followed by washes with 95% ethanol and 80% ethanol. Whole-genome sequencing was achieved in a 6-plex library using a long-read Pacific Biosciences (PacBio) single-molecule real-time (SMRT) sequencing platform.

Results

Conclusion