Abstract
ABSTRACTNitrosomonas cryotolerans ATCC 49181 is a cold-tolerant marine ammonia-oxidizing bacterium isolated from seawater collected in the Gulf of Alaska. The high-quality complete genome contains a 2.87-Mbp chromosome and a 56.6-kbp plasmid. Chemolithoautotrophic modules encoding ammonia oxidation and CO2 fixation were identified.
Highlights
Nitrosomonas cryotolerans ATCC 49181 is a cold-tolerant marine ammoniaoxidizing bacterium isolated from seawater collected in the Gulf of Alaska
Cultures were harvested by centrifugation, and genomic DNA was isolated using the Joint Genome Institute (JGI) cetyltrimethylammonium bromide (CTAB) protocol
The quality of genomic DNA (gDNA) was checked by gel electrophoresis and by amplification and sequencing of the V4 region of the small subunit (SSU) rRNA gene [5]
Summary
Nitrosomonas cryotolerans ATCC 49181 is a cold-tolerant marine ammoniaoxidizing bacterium isolated from seawater collected in the Gulf of Alaska. Nitrosomonas cryotolerans was grown in marine medium containing 25 mM ammonium at 15°C [4]. Cultures were harvested by centrifugation, and genomic DNA (gDNA) was isolated using the JGI cetyltrimethylammonium bromide (CTAB) protocol. The quality of gDNA was checked by gel electrophoresis and by amplification and sequencing of the V4 region of the small subunit (SSU) rRNA gene [5].
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