Complete Genome Sequence of Nitrogen-Fixing Paenibacillus sp. Strain URB8-2, Isolated from the Rhizosphere of Wild Grass.

Kyosuke Kita,Shu Ishikawa,Ken-Ichi Yoshida,J Cameron Thrash

doi:10.1128/mra.00814-20

Abstract

We report here the complete genome sequence of nitrogen-fixing Paenibacillus sp. strain URB8-2, isolated from the rhizosphere of wild grass in Kobe, Japan, revealing that this bacterium is related to Paenibacillus rhizophilus 7197, a novel species collected recently in Inner Mongolia, China, and that it possesses two gene clusters for distinct types of nitrogenases.

Highlights

URB8-2 was grown in tryptic soy broth (TSB) (Becton, Dickinson and Company) at 30°C for 48 h with shaking at 200 rpm
The NEBNext Ultra II FS DNA library prep kit for Illumina (New England BioLabs) was used to prepare a genomic DNA (gDNA) library enriched for DNA fragments around 1,000 bp for Illumina sequencing
The findings suggested that URB8-2 belongs to the genus Paenibacillus

Summary

Introduction

URB8-2 was grown in tryptic soy broth (TSB) (Becton, Dickinson and Company) at 30°C for 48 h with shaking at 200 rpm. The genomic DNA (gDNA) was isolated using conventional phenol-chloroform extraction (2). The NEBNext Ultra II FS DNA library prep kit for Illumina (New England BioLabs) was used to prepare a gDNA library enriched for DNA fragments around 1,000 bp for Illumina sequencing. (2 ⫻ 300 bp) was carried out using the MiSeq platform with the MiSeq reagent kit v3 (Illumina). The raw paired-end reads were trimmed using Sickle v1.33 (3) to remove low-quality (quality [Q] score, ⬍25) reads and short reads (⬍75 bp).

Results

Conclusion