Abstract
Group B Streptococcus (GBS) is an asymptomatic colonizer of the female reproductive tract but can cause maternal and neonatal infections and adverse pregnancy outcomes. Here, we closed the genome sequence of strain CJB111, a neonatal GBS clinical isolate from a case of late-onset bacteremia without focus (Houston, TX; 1990).
Highlights
The Gram-positive beta-hemolytic bacterium Streptococcus agalactiae asymptomatically colonizes the gastrointestinal and female genital tracts of healthy adults but can cause neonatal infections and adverse pregnancy outcomes [2]
CJB111 was grown statically overnight at 37°C in Todd Hewitt broth (THB), genomic DNA was purified (Gentra PureGene Yeast/Bact kit), and concentration and quality were confirmed by NanoDrop spectroscopy
Hybrid assembly via Unicycler v0.4.8 with a verbosity value of 2 [13] yielded six contigs, which were further assembled into three nonoverlapping contigs upon mapping to CJB111 contigs (AAJQ01000000) in Geneious v11.1.5 [14]
Summary
Complete Genome Sequence of Neonatal Clinical Group B Streptococcal Isolate CJB111. Dorana aDepartment of Immunology and Microbiology, School of Medicine, University of Colorado-Anschutz Medical Center, Aurora, Colorado, USA bDepartment of Pediatrics, McGovern Medical School, University of Texas Health Science Center, Houston, Texas, USA cChildren’s Memorial Hermann Hospital, Houston, Texas, USA
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