Abstract
Mycoplasma felis causes conjunctivitis in cats and respiratory diseases in horses. We report here the complete genome assembly of equine Mycoplasma felis strain Myco-2, which was isolated from an ill horse in Japan.
Highlights
Mycoplasma diseases are relatively uncommon in horse populations, but Mycoplasma felis is known to cause equine respiratory diseases
Short reads generated by Ion Personal Genome Machine (PGM) were trimmed in Sickle v. 1.33 software [6] using a quality threshold of 27 and a length threshold of 100 bp
The same genomic DNA was used for nanopore sequencing, and a library was prepared with a ligation sequencing kit (SQK-LSK109; Oxford Nanopore Technologies [ONT]) and a native barcoding expansion kit (EXP-NBD104; ONT)
Summary
Mycoplasma diseases are relatively uncommon in horse populations, but Mycoplasma felis is known to cause equine respiratory diseases. Short reads generated by Ion PGM were trimmed in Sickle v. 1.33 software [6] using a quality threshold of 27 and a length threshold of 100 bp.
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