Abstract
Micrococcus luteus strain SGAir0127 was isolated from indoor air samples collected in Singapore. The assembly, based on single-molecule real-time sequencing reads, resulted in two contigs, one chromosomal contig with a length of 2.57 Mbp and one nonchromosomal contig of 8.68 kbp. The genome has a total of 2,564 genes.
Highlights
Micrococcus luteus strain SGAir0127 was isolated from indoor air samples collected in Singapore using a single-stage Andersen impactor (SKC, USA) to impact air onto a Trypticase soy agar (TSA) plate (Becton, Dickinson, USA)
Phyla-AMPHORA was run using MarkerScanner.pl with added -DNA flag and MarkerAlignTrim.pl and with options -WithReference and -OutputFormat phylip, followed by Phylotyping.pl, with default parameters [9]. It was used for taxonomic identification and showed that the assembled genome has 99.5% marker identity with Micrococcus luteus
The results showed 97.8% identity to Micrococcus luteus NCTC 2665 with an alignment fraction of 0.60
Summary
Micrococcus luteus strain SGAir0127 was isolated from indoor air samples collected in Singapore (global positioning system [GPS] coordinates 1.3451N, 103.6789E) using a single-stage Andersen impactor (SKC, USA) to impact air onto a Trypticase soy agar (TSA) plate (Becton, Dickinson, USA). The Hierarchical Genome Assembly Process (HGAP) version 3 (with default values) [7] from PacBio’s SMRT Analysis 2.3.0 package assembled a total of 60,672 reads into two contigs. After Quiver polishing (with default values) [7], a chromosomal contig with a length of 2,570,740 bp and 201-fold coverage and a nonchromosomal contig with a length of 86,829 bp and 207-fold coverage were found.
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