Complete Genome Sequence of Methanobacterium electrotrophus Strain YSL, Isolated from Coastal Riverine Sediments.

Shiling Zheng,Fanghua Liu,Frank J Stewart

doi:10.1128/mra.00752-21

Abstract

ABSTRACTMethanobacterium electrotrophus strain YSL was isolated from enriched microbial aggregates from a coastal riverine sediment sample from Shandong Province, China. The genome of YSL was sequenced with the PacBio Sequel platform and contained three plasmids in addition to the chromosome. A total of 2,521 protein-coding genes and 58 RNA genes were predicted.

Highlights

Methanobacterium, the genus of hydrogenotrophic methanogens, is widely distributed in coastal riverine sediments [1,2,3]
A-tailing and sequencing adapter ligation of fragments were performed, and a qualified DNA library was prepared for complete genome sequencing with the PacBio Sequel single-molecule real-time (SMRT) sequencing technology (Pacific Biosciences)
After quality filtering of raw data using MECAT2 [5] with default settings, 62,441 reads, with an N50 value of 12,977 bp, were assembled using SMRT Link v5.1.0 to generate 4 contigs, with a maximum contig length of 2,509,437 bp (Table 1)

Summary

Introduction

Methanobacterium, the genus of hydrogenotrophic methanogens, is widely distributed in coastal riverine sediments [1,2,3]. Genomic DNA of M. electrotrophus strain YSL was extracted according to the manufacturer’s instructions for a bacterial DNA extraction minikit (Onrew, China). The quality and concentrations of DNA extracts were determined by standard gel electrophoresis and analysis with a Qubit fluorometer (Invitrogen, USA).

Results

Conclusion